3D-printed stamp for standardized mounting and high content confocal imaging of (zebrafish) embryos
Automation, high content, confocal, mounting, (zebra-)fish
Improved specimen preparation and well-plate like sample navigation for a more efficient setup of automated multi-dimensional imaging process of live or fixed zebrafish embryos in inverted and motorized stage microscope settings.
- Increased sample size
- Standardized specimen arrangement, allowing for automated imaging solutions
- Optimized spatial arrangement in Z, allowing for a reduction of Z-planes in confocal imaging
- Soft embedding, allowing for embryo growth when live imaging
- User-friendly easy setup
Figure 1 Stamping models and mounted embryos. (A-B) Stamp surfaces of (A) Lateral mounting and (B) Dorsal mounting stamp (C) Ready-to-use agarose imprint with 44 µ-wells. (D) Mounted embryos ready for imaging.
Figure 2 Stamp and µ-well proportions (A, A’) Schemes showing a zebrafish embryo mounted without µ-well (A) and with µ-well (A’). (B, B’) Top-view (B) and orthogonal view (B’) of a single micro well. (C) Design and dimensions of the stamp. The base connects all parts and fits exactly a 20 mm dish. and are the distances in X and Y. Stabilizers were introduced as cornerstones and to make the structure more rigid. (D) For better handling, the stamp is mounted onto a countersunk screw.