Method and Device for Fluorescence Investigations of fixed and living Three Dimensional Samples

Method and Device for Fluorescence
Investigations of fixed and living Three
Dimensional Samples 

Partnership opportunities

We are seeking partners for:

-Collaborative development

-Technology licensing

-Further validation and breeding-oriented development

 

For Contact:

Technology Transfer:
INNOVECTIS GmbH
 Altenhöferallee 5
 60438 Frankfurt am Main, Germany
 Phone: +49 69 25 61 632-0
 matthias.goetz@innovectis.de

Technology

This technology is based on light sheet fluorescence microscopy (LSFM) for the investigation of fixed and living three-dimensional biological samples with high temporal resolution.

A specially developed capillary sample holder, combined with optical clearing, enables aberration-free fluorescence excitation, continuous adjustment of the light-sheet focal plane, and imaging from different angles by rotating the sample holder.

Applications

-The method is suited for 3D imaging of biological samples such as cell cultures, embryos, and tissue, including sensitive living specimens, living plants, and fixed or cleared probes.

-It also supports controlled sample environments and can be combined with microfluidic perfusion systems to add drugs or other agents during observation, which makes it attractive for miniaturized setups and high-throughput screening.

Technology stage

-A prototype of the invention has already been designed and tested.

Patents and Puplications

-The patent portfolio includes DE 10 2012 108 1 B584, EP 2 893 319 A1, US 2015/0211981 A1, and JP 2015-534049 A.

-Pampaloni, F., Ansari, N. & Stelzer, E.H.K. High-resolution deep imaging of live cellular spheroids with light-sheet-based fluorescence microscopy. Cell Tissue Res 352, 161–177 (2013). https://doi.org/10.1007/s00441-013-1589-7 
-I. Smyrek and E. H. K. Stelzer, “Quantitative three-dimensional evaluation of immunofluorescence staining for large whole mount spheroids with light sheet microscopy,” Biomed. Opt. Express 8, 484-499 (2017)